Name _________________________________

DNA Fingerprinting

DNA

Site: PBS / NOVA - go to http://www.pbs.org/wgbh/nova/ , click on Nova Teachers, then go to Life Science and find the activity on creating a DNA fingerprint. Direct link: http://www.pbs.org/wgbh/nova/teachers/body/create-dna-fingerprint.html. You can also search for this using google by searching for "create a dna fingerprint"

Objective: Students will learn the steps of DNA fingerprinting by creating a fingerprint in a virtual lab. They will use this fingerprint to solve a virtual crime. The virtual lab is interactive and goes through the step-by-step process of DNA fingerprinting

1. What crime was committed?

2. Who are the suspects?

Creating a DNA Fingerprint

3. What is Step 1?

4. What does a restriction enzyme do to DNA?

5. What is Step 2?

6. Describe agarose:

7. What is Step 3?

8. What is Step 4?

9. What is electrophoresis?

10. Which fragments of DNA will move the farthest?

11. What is Step 5?

12. What is the purpose of the nylon membrane?

13. What is Step 6?

14. How are the probes labeled?

15. What is Step 7?

16. Analyze the DNA. Which one of the suspects is the culprit?

Site 2: Go to DNA.gov

17. Browse the website until you get a good idea what the purpose of the site is. In your opinion, what is DNA.gov being used for?

18. DNA can be used to solve crimes, but the government also uses it for what other purposes?

19. Name a course that is offered at this site.

20. Look under statistics and find how many offender profiles are stored in your state's database. What is a database?