Site: PBS / NOVA - go to http://www.pbs.org/wgbh/nova/ , click on Nova Teachers, then go to Life Science and find the activity on creating a DNA fingerprint.
Objective: Students will learn
the steps of DNA fingerprinting by creating a fingerprint in a virtual lab.
They will use this fingerprint to solve a virtual crime. The virtual lab
is interactive and goes through the step-by-step process of DNA fingerprinting
1. What crime was committed? lollipop was licked
2. Who are the suspects? 7 sisters
Creating a DNA Fingerprint
3. What is Step 1? pour restriction enzymes
4. What does a restriction enzyme do to DNA? cuts it
5. What is Step 2? pour agarose gel
6. Describe agarose: like jell-o
7. What is Step 3? pour DNA
8. What is Step 4? push power
9. What is electrophoresis? moving molecules with electric current
10. Which fragments of DNA will move the farthest? smallest
11. What is Step 5? place nylon membrane
12. What is the purpose of the nylon membrane? for moving, gel is difficult to work with
13. What is Step 6? add probes
14. How are the probes labeled? radioactive
15. What is Step 7? place x ray film
16. Analyze the DNA. Which one of the suspects is the culprit? Honey
Site 2: Go to DNA.gov
17. Browse the website until you get a good idea what the purpose of the site is. In your opinion, what is DNA.gov being used for?
solving crimes, training law enforcement, storing data (lots of different answers)
18. DNA can be used to solve crimes, but the government also uses it for what other purposes? identify remains, clear people who have been convicted of crimes
19. Name a course that is offered at this site. answers vary
20. Look under statistics and find how many offender profiles are stored in your state's database. answers vary, Illinois has 401,218
What is a database? place where data is collected and stored