DNA Replication
Label: DNA polymerase 3’ 5’ DNA Ligase Okazaki fragment DNA Primase
Single Strand Binding Proteins Helicase Leading Strand Lagging Strand
Identify the structure
1. _____________________________ Enzyme that unwinds DNA
2. _____________________________ Fragments of copied DNA created on the lagging strand
3. _____________________________ The strand that is copied in a continuous way, from the 3’ to 5’ direction
4. _____________________________ Binds Okazaki fragments
5. _____________________________ Builds a new DNA strand by adding complementary bases
6. _____________________________ Stabilizes the DNA molecule during replication
7. _____________________________ Strand that is copied discontinuously because it is traveling away from helicase
8. _____________________________ Initiates the synthesis DNA by creating a short RNA segment at replication fork
9. Place the events in the correct order:
____ DNA polymerase adds nucleotides in the 5’ to 3’ direction
____ Replication fork is formed
____ DNA polymerase attaches to the primer
____ Okazaki fragments are bound together by ligase
____ DNA helicase unwinds DNA
10. Why is replication called “semi-conservative?” ____________________________________