Enzyme Lab - Teachers Guide

Original Document: Enzyme Lab

MATERIALS: The chemicals can be purchased from chemical or biological supply companies, students should wear safety goggles This lab requires some set up and can be messy. Not advised unless you actually have a lab to work within.

MATERIALS: 6 Test tubes Test tube holders 3% Hydrogen peroxide

Straight-edged razor blade  Scissors and Forceps Measuring Pipettes Stirring rod

Fresh liver, Apple, and Potato, Yeast Vinegar / Baking Soda HCL and NaOH pH paper (optional)

Ice bath Warm water bath Boiling water bath

The majority of the synthesis questions use the Claim - Evidence - Reasoning model. This rubric can be used to grade each of them. I print small label stickers to projects to make grading quick. Students in my class are used to this model by the time we do this lab.

Label Version (Print on Labels for exams)

Claim (accurate, complete, grammar)  __3__2__1
Evidence (appropriate, sufficient, data)  __3__2__1
Reasoning (links to claim and sci. principles) 3__2__1

I do not have the answers to the questions (key) but generally speaking..

• catalase (all enzymes) are reusuable
• almost all eukaryotic cells have peroxisomes, so all living tissues (of eukaryotes) have the enzymes
• raising temperature will increase enzyme activity
• catalase seems to work best in a basic environment, too much base (or acid) will denature the protein

It is more important that students provide evidence from their experiment to support their claims.

CLAIM-EVIDENCE-REASONING (RUBRIC)

	Accomplished (3)	Proficient (2)	Developing (1)
CLAIM	Makes an accurate and complete claim, uses complete and grammatically correct sentences	Makes an accurate, but incomplete claim, or grammatically incorrect	Claim is not accurate, incomplete, or unintelligible
EVIDENCE	Provides appropriate and sufficient evidence to support claim by referencing specific data, observations, or text evidence (for readings)	Provides appropriate data but insufficient data, too general or lacking in details	Provides evidence but it is insufficient, inaccurate, no details
REASONING	Provides thorough reasoning that links evidence to the claim, references scientific principles that are relevant to claim	Provides reasoning that links evidence to the claim, lacks scientific principles	Does not provide reasoining, or reasoning does not link evidence.